University of Wisconsin Madison
Microscopy of Life (B M E 619) Syllabus
Course Learning Outcomes
    Course Learning Outcome
  • 1
    Understand the principle and capabilities of the microscopy and biomedical imaging modalities covered in the class.
  • 2
    Read and assimilate current literature papers on these topics
  • 3
    Write an informed paper on these topics.
  • 4
    Understand how these techniques could be applied to the students’ own research.
Microscopy of Life
B M E 619 ( 3 Credits )
Survey of state of the art microscopic, cellular and molecular imaging techniques, beginning with subcellular microscopy and finishing with whole animal imaging.
2nd semester intro physics including light & optics (e.g. 104, 202, 208) or cons inst
College: College of Engineering
Instructor Name
Instructor Campus Address
Contact Hours
Course Coordinator
Text book, title, author, and year
Supplemental Materials
Papers from literature
Required / Elective / Selected Elective
Selected Elective 
ABET Program Outcomes Associated with this Course
Program Specific Student Outcomes
(1) Understanding of biology and physiology as related to biomedical engineering needs.
Brief List of Topics to be Covered

Biological Imaging is a vast field ranging from single molecule imaging, electron microscopy, live cell imaging and fluorescence approaches to medical and diagnostic imaging techniques. In this survey class we will review many of the fundamental aspects of biological microscopy and biological and biomedical imaging in general. Students will be exposed to biological imaging research from across the UW and in lab tours take part in imaging and computational demonstrations. It is the goal that at the end of this "Microscopy of Life" course, given a real-research imaging or micro-analytical problem in biology or medicine, a successful student will be able to identify which technique is best suited to answer a variety of biological or physiological questions. The course is designed to be a lead-in for more advanced imaging subject matter courses at the UW such as “BME 601-Special Topics in Biological Microscopy”, “Med Phys 530-Medical Imaging Systems” and “Med Phys 471-Multimodality Molecular Imaging in Living Subjects”.

Topics include:

  • Live cell microscopy and confocal
  • Multiphoton and second harmonic generation
  • Imaging in cancer
  • Biological Electron Microscopy (SEM, cryo, TEM, ET)
  • FRET
  • Bio-inspired optics endoscopes, adaptive optics
  • IR spectromicroscopy
  • Optogenetics and Neuro Stimulation
  • Image Informatics Introduction
  • Optical Mapping
  • MicroPET
  • Micro CT
  • Functional MRI
  • Ultrasound and bioacoustic microscopy
Additional Information
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